Effect of adding gelatin and its modified form on properties of enzymatically-gellable alginate hydrogels for soft tissue engineering applications

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 336

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شناسه ملی سند علمی:

ITERMED01_299

تاریخ نمایه سازی: 7 مرداد 1398

چکیده مقاله:

Introduction Alginate is a natural polysaccharide that has many applications in medical fields for its excellent biological compatibility and biodegradation. However, this substance lacks cellular interactions and has poor cell adhesion. Objectives In our study, the phenolic moieties were introduced to alginate (EDC / NHS) to form enzymatically-gellable alginate (Alg-ph) for mechanical strength improvement in culture medium and gelatin was also used to enhance the cellular adhesion properties. Methods In order to prevent gelatin from leaving the scaffold, this compound was also phenolized using EDC / NHS (Gel-ph). Hydrogels of Alg-ph, Alg-ph + gel and Alg-ph + gel-ph were then prepared using Horsradish peroxidase (HRP) and H2O2 and analyzed by UV, swelling, mechanical strength and cell culture test. Results Both modified Alginate and modified gelatin showed absorption at 275 nm wave lengths, indicating that phenolic groups were successfully introduced on the compounds. By adding gelatin to Alg-ph, the swelling and strains of hydrogel increased 3.5 and 5-fold respectively. Also, with the addition of modified gelatin compared to gelatin, swelling increased about 5.5 times and the breaking strain was reduced 0.8 times due to the porosity created in the hydrogel structure. Evaluation of chondrocyte cell proliferation within Alg-ph + gel, Alg-ph + gel-ph hydrogels revealed 3.7 and 4 fold increasing for 21 days respectively. Conclusion According to the observed results, Alg-ph + gel-ph is an appropriate scaffold for use in soft tissue engineering, especially for cartilage tissue.

نویسندگان

Sahar Kiani Golshouri

Tissue engineering and stem cell research Lab, Chemical Engineering Faculty, Sahand University of Technology, Tabriz, Iran

Ali Baradar Khoshfetrat

Tissue engineering and stem cell research Lab, Chemical Engineering Faculty, Sahand University of Technology, Tabriz, Iran