The study of the effect of shilajit hydro methanolic extract on differentiation of human mesenchymal cell osteoblast

IntroductionShilajit is the substance is black-brown in color, is found in openings and fractures adjacent to the underground oil reserves and the height of the mountains. Traditionally it has been used to treat fractures.ObjectivesIn the present study, we investigated the effect of Shilajit Hydro methanolic extracton mesenchymal stem cells and their differentiation into osteoblast. Mesenchymal stem cell, supporting hematopoetic stem cells in bone marrow, can regenerate tissues such as bone, cartilage, muscle, tendon and fattytissue.MethodsIn this experimental study, bone marrow cells were prepared from Pasteur Institute and were treated with 150 and 250 μm concentration of Shilajit. cytotoxicity of Shilajit extract was measured by MTT assay (3-4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide). Differentiation effectswere investigated by alizarin red stainingafter 21 daysand alkaline phosphates (ALP) activity after 14 days.ResultsThe results showed after 24 hours, cell proliferation was significantly increased and did not have any toxic effectson the cells. Alkaline phosphatase activity was more than control in the treatment, indicating the onset of differentiation in the cells. Alizarin red staining showed Shilajit Hydro methanolic extractincreased osteogenic differentiation.ConclusionAccording to the findings of this study, used of Shilajit Hydro methanolic extract effectively enhance BMSCs differentiation

کلیدواژه ها:

Mesenchymal stem cells ، Osteoblast ، Shilajit ، Differentiation