Impact of polysaccharide based derivative hydrogel microcapsule membrane on encapsulated cells

Introduction The hydrogel-based microcapsules having the ability to promote cell adhesion, proliferation and differentiation are useful tool in fabricating tissue in vitro. Objectives In this study, we investigate the impact of the two anionic polysacharide hydrogel membranes which has impact on the cellular adhesiveness, morphology and growth of cells. Methods The hyaluronic acid and alginate possessing phenolichydroxyl moieties (HA-Ph and Alg-Ph respectively) microcapsules were produced through the horseradish peroxidase-catalyzed reaction using temporal gelatin microparticle. Morphological and functional characteristics of encapsulated cells were evaluated intensively.ResultsThe results confirm the formation of amide bonds due to the interaction of free carboxyl and amino group present in HA-based hydrogel and gelatin, respectively that provide a superior microenvironment for attachment and growth of encapsulated and seeded cells. The HA-Ph hydrogel ionically crosslinked with gelatin was shown highest degree of cytocompatibility regarding cell adhesion, spreading, and proliferation compared with Alg-Ph hydrogel membrane. Conclusion These findings indicated the HA-Ph microcapsule encomposed temporal gelatin microparticle is a promising substrate in tissue engineering applications

کلیدواژه ها:

Cell encapsulation ، Cell adhesion ، Anionic polysaccharide-based hydrogel ، Electrostatic interaction ، Tissue engineering