Synthsis of electroactive hydrogel scaffold based on fibrin and tetraniline crosslinked by genipin

Introduction Hydrogels can mimic the nature of tissue from the physical, chemical, electrical, and biological properties, and thus provide an appropriate microenvironment for cell growth and drug release. The electroactivity exerts great inflence on cell activities and endows hydrogels with electricalstimuli response. Objectives The aim of this study was to synthesize the electroactive hydrogel scaffold based on fibrin and tetraaniline and evaluate the electrical and physicochemical properties. Methods Aniline tetramer (AT) was prepared with a surface polymerization. 0.5 mmol of genipin (Gen) solution was added to AT solution to make cross-linked between AT and fibrin. The mixture was added to 15 μl of thrombin solution. On the other hand, 1.5 mg of fibrinogen was dissolved in 0.5 mL DMEM solution and then transferred to 24-well culture dish. Afterward, thrombin mixture and 50 μl of fetal bovine serum were added to fibrinogen solution to form a gel. The chemical structure and morphology of the hydrogel and AT were analyzed by FTIR. The conductivity of the AT was evaluated by cyclic voltammetry (CV) and UV-Vis spectroscopy. Results The chemical structures were confirmed by FTIR. The electroactivity of the gel was confirmed by the UV spectra and CV. Conclusion We have prepared electroactive hydrogel scaffold based on fibrin and tetraaniline. The results indicated that this hydrogel possessed excellent electrical activity which can be a promising candidate for cardiac or nerve tissue engineering.

کلیدواژه ها:

Fibrin hydrogel ، Tetraaniline ، Electroactive hydrogel