Fabrication of quaternized cellulose electrospun nanofiber and evaluation of its antibacterial effects and biocompatibility

Needs for new biomaterial with suitable properties to achieve cell viability and proliferation. Cellulose as a most abundant biopolymer in the earth has the potential to be used in this area. Due to three reactive hydroxyl groups, different derivatives of cellulose can be synthesized. Among different type of cellulose, nanofabricated cellulose due to their ability to mimic the extracellular matrix in topography and chemistry becoming popular as scaffolds. In this work, we reported the fabrication of antibacterial nanostructured cellulose based scaffold for biomedical applications. The cellulose acetate nanofibers were electrospun and followed by deacetylation to produce cellulose nanofibers. Quaternized cellulose derivative was synthesized by reacting cellulose nanofibers with 3-chloro-2-hydroxypropyltrimethylammonium chloride in alkali solution. The effects of these modifications on the structure and surface properties were characterized by SEM, ATR-FTIR, Zeta potentiometry, mechanical strength, water uptake and wettability. Disk diffusion method and MTT assay were carried out to determine antibacterial effects and biocompatibility of fabricated nanofibers. FTIR results indicated that quaternized cellulose nanofibers have an obvious new peak at 1491 cm−1 compared to cellulose nanofibers which attributed to quaternary ammonium groups. Fabricated electrospun nanofibers had 100-600 nm diameter, +22 mV surface potential, 7 and 482 MPa tensile strength and Young module, 640% water uptake and 470 contact angle. Quaternized cellulose nanofibers showed strong antibacterial activity toward both gram positive and gram negative bacteria and cell culture experiments demonstrated that the scaffold was quite biocompatible for human mesenchymal stem cells attachment and proliferation.

کلیدواژه ها:

Antibacterial ، Biocompatible ، Cellulose nanofiber ، Electrospun ، Quaternization