Comparison of cell attachment and proliferation between microfibrous and nanofibrous carboxymethyl cellulose scaffolds

IntroductionFibers are continuous structure materials that have an extremely high ratio of length to width. Over the past few decades, uses of fibrous biomaterials have met with success in tissue engineering and regenerative medicine since fibrous structure can morphologically resemble extracellular matrix. In comparison to synthetic materials, cellulose based fibers have interesting properties such as biodegradability and biocompatibility. The use of this material in biomedical applications has gained attention due to the multifunctionality of cellulose, for example, the possibility of chemical and physical alternations. Carboxymethyl cellulose (CMC) is one of the cellulose derivatives that have the potential to be used in tissue engineering.MethodsIn this work, we reported the fabrication of microfibrous and nanofibrous CMC scaffolds and comparison of adhesion and spreading potential of human mesenchymal stem cells (hMSCs) on electrospun CMC nanofibers and microfibrous one. The fabricated fibrous scaffolds were characterized by SEM, FTIR, Zeta potentiometry and their water uptake, wettability and mechanical strength were measured.ResultsFTIR results indicated that carboxymethyl cellulose fibrous scaffolds have an obvious new peak at 1595 cm−1 compared to cellulose fibers which indicate the success of the carboxymethylation. The results showed nanofibrous CMC has higher water uptake, wettability and mechanical strength than microfibrous one. The MTT assay showed that proliferation of hMSCs is enhanced in nanofibrous CMC, compared to the microfibrous scaffold. The SEM of fixed hMSCs on scaffolds showed average length of cells with nanofibers is significantly longer than of the cells with microfibers,ConclusionThe nanofibrous scaffolds may enhance the cell outgrowth

کلیدواژه ها:

Carboxymethyl cellulose scaffold ، Cell attachment ، Microfibrous ، Nanofibrous ، Proliferation