Modifying mesenchymal stem cell sheet bio-engineering using thermo-responsive hydrogel copolymer and antioxidant

ObjectivesThe aims of this study was modifying and comparing of AT-MSCs sheet formation and detachment using temporary scaffold which was thermo-sensitive hydrogel based on poly N-isopropyl acryl amide-Methacrylic acid P(NIPAAm-MAA) copolymer.MethodsThermo- responsive P(NIPAAm-MAA) copolymer was synthesized and characterized by H-NMR, FTIR. The cell sheet fabrication was performed, subsequently the SEM imaging, H & E staining and senescence of released cell sheet was studied.ResultsWe found that AT-MSCs have the potential for sheet production on thermo-responsive P(NIPAAm-MAA) copolymer, also using MAA increased LCST point of polymer, therefore detachment of sheet didn’t require more than 2ºC thermal reduction from 37 ºC (LCST=35). SEM and H&E staining showed that using ascorbic acid enhance integrity and quality of cell sheet and also it causes high level of ECM secretion too. The fabricated sheets exhibited osto/adipogenic differentiation potential. Also the cell sheet that treated by ascorbic acid showed less senescence.ConclusionWe concluded that, using P(NIPAAm-MAA) could facilitate the detachment and prevented damage to the engineered AT-MSCs sheet by a sol to gel transition at a temperature near to physiologic conditions. Also using ascorbic acid as an antioxidant improved cell sheet quality and integrity

کلیدواژه ها:

Cell sheet ، AT-MSCs ، P (NIPAAm-MAA) ، Ascorbic acid