Detection of Lead and Cadmium using Biosensor Expressing Luciferase Reporting Gene in Aquatic Environment

سال انتشار: 1398
محل انتشار: سیزدهمین کنگره ی پژوهشی سالیانه دانشجویان علوم پزشکی شرق کشور
کد COI اختصاصی: MSEMSMED13_112
زبان مقاله: انگلیسی
تعداد مشاهده: 546

نویسندگان

Student research committee, Islamic Azad university, Mashhad Branch, Mashhad, Iran

Department of Medical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

چکیده

Background and Objective: Bacterial biosensors refer to bacterial cells that have been genetically engineered to produce a measurable signal in response to a specific chemical or physical agent in their environment. Bacterial biosensors for detection of heavy metals typically composed of a promoter (responsible for sensing or interacting with the target analyte) fused to a reporter gene (responsible for generation of the detectable biological signal). Biosensor has great potential for detection of environmental contaminants. Method and material: Two biosensor gene constructs were engineered by using lead responsive elements from plasmid pMOL30 of Ralstonia metallidurans CH34 strain (pbr promoter and pbrR as a regulatory gene) and lead and cadmium responsive elements from pI258 plasmid of Staphylococcus aureus (cadA promoter and cadC as a regulatory gene) to control the expression of the firfly luciferase gene, which functioned as the reporter. They were called pGL3-luc/pbr-Biosensor and pGL3-luc/Cad-Biosensor respectively. Escherichia coli DH5α is the host organism. Results: pGL3-luc/pbr-Biosensor is reported to be able to detect Pb+2 in the range of 1–100 μM by expressing firefly luciferase and not expressing reporter in present of other metals such as Sn+2, Ni+2, Cd+2. Moreover, this biosensor is 50 times more sensitive compared to the previous biosensors constructed by Chakraborty. Conclusion: Use of these bacterial biosensors has many benefits such as measuring the available concentrations of heavy metals, saving time and cost and ease of use.

کلیدواژه ها

bacterial biosensor, pbr promoter, cadA promoter, heavy metals, contamination, detection, lucifer

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