Effect of Triiodothyronine on Remyelination Enhancement After Mesenchymal Stem Cell Intraperitoneal Injection in Cuprizone Model of Multiple Sclerosis.

Background and Objective: Multiple sclerosis (MS) is one of the most important inflammatory diseases of the central nervous system, yet its etiology is not well defined. Cell therapy for MS has become one of the newest therapies, even in its clinical phase. We used thyroid hormone subcutaneously to enhance effects of the transplanted stem cells for remyelination in corpus callosum. Materials and Method: First, human bone marrow mesenchymal stem cells (BM-MSCs) are cultured with α-MEM containing 10% FBS. Demyelination model was induced in mice for 6 weeks by administration of the cuprizone, and in the fourth and fifth week after the first dose of Cuprizone, the stem cells were administered intraperitoneally to the mice. From the fourth week to the end of the sixth week, the thyroid hormone was subcutaneously injected into the mouse. At the end of the sixth week, the rats were killed and subjected to tissue evaluations using the LFB staining technique for myelin pods also MBP and PDGF-α receptor immunohistochemistry.The expression of MBP, TR-β1, TR-β2 genes was investigated by realTime-PCR. findings: In histology studies, demyelination has been induced by cuprizone, and this is clearly evident in the luxol fast blue staining and immunohistochemistry and molecular techniques. In the Luxol fast blue and Immunohistochemistry technique for MBP and PDGF-αR increased recovery in the combination group with the cell and the T3 hormone was seen compared to other groups. In the molecular studies, the combination group did significantly increase the expression of the MBP, TR-β1 and TR-β2 genes. However, TR-β2 gene played a more significant role. Conclusion: In this project, a combination therapy strategy has been used to accelerate myelination in the mice cuprizone model. We used sequential injection of thyroid hormone simultaneously and repeated injection of the stem cells for two consecutive weeks to accelerate the process. In groups where we used combined cell and thyroid injection, we observed an increase in myelination compared to other groups that we used only from cells or hormone

کلیدواژه ها:

Department of Neuroscience ، School of Advanced Technologies in Medicine ، Tehran University of Medical Sciences ، Tehran ، Iran.