Survey the prevalence of plasmid-dependent beta-lactamase resistance genes in gram-negative bacteria(Acinetobacter, Proteus, Klebsiella and Escherichia coli) commonly present in Amir al-Momenin Burns Hospital in Shiraz

Introduction: The producing ESBL enzymes, is the most important resistance factor against ß-lactam antibiotics among gram-negative bacteria family. The goal of this study was to investigate the prevalence of plasmid-dependent beta-lactamase resistance genes in gram-negative bacteria generally reported in Amir-al-Momenin Hospital.Methods: In a one-year period (2016-2017), 180 clinical specimens were collected from the hospital. After phenotypic identification, the samples were extracted using alkaline lysis with SDS plasmid. By using specific primers, the presence plasmid gens of tem, shv, pse, oxa10, ges, and kpc were investigated by PCR method. Data were analyzed using SPSS 22 software.Results: Of 180 gram-negative bacteria isolated, 72(40%) Acinetobacter, 1(0.6%) Klebsiella, 88(48.9%) Pseudomonas aeruginosa, 1(0.6%) Proteus, 2(1/1%) Escherichia coli and 16 (8.9%) were other gram-negative bacteria. The isolated samples included 33(43.4%) wound, 23(30.3%) urine, 10(13.2%) tissue, and the remaining specimens included throat and nose swabs. Of the obtained, 76 samples including 4 bacteria were investigated in this study, which included 51(67.1%) males and 25(32.9%) females with a mean age of 28.25±7.91 years. The presence of plasmid-dependent Extended-spectrum beta-lactamase genes was 24(31.6%) tem, 1(1.3%) ges, 47(61.8%) shv, 10(13.2%) oxa10, 16(21.1%) pse and (0%) kpc were obtained.Conclusion: With increasing drug resistance and confirmation of the presence of ESBLS and multi-drug resistance in pathogens, regular molecular monitoring of these genes in burn patients and other patients related to hospitals in the country is recommended.