Inhibitory Effect of Nano-gel/Emulsion of Chitosan Coating Incorporated with Ziziphora Clinopodioides Essential Oil and Nisin on Escherichia Coli O157:H7 Inoculated in Beef at Cold Storage Condition
- سال انتشار: 1398
- محل انتشار: فصلنامه تغذیه، روزه داری و سلامت، دوره: 7، شماره: 2
- کد COI اختصاصی: JR_JNFH-7-2_007
- زبان مقاله: انگلیسی
- تعداد مشاهده: 611
نویسندگان
Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Iran.
Department of Food Hygiene and Aquatics, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Iran.
Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Clinical Sciences. Faculty of Veterinary Medicine. Ferdowsi University Of Mashhad, Mashhad, Iran
چکیده
Introduction: This study was conducted to evaluate the influence of chitosan Nano-gel/emulsion coating functionalized by Ziziphora clinopodioides essential oil (ZCEO) and nisin on growth inhibition of Escherichia coli O157: H7 inoculated in beef samples during 16 days in cold storage condition (4˚C). Methods: Beef sample was divided into six groups after inoculation of E. coli O157:H7. Treatments including control (no coating), chitosan 2%, sonicated chitosan 2%, Nano-emulsion of chitosan coating containing ZCEO (0.5%), Nano-gel of chitosan coating containing nisin (200 IU/g), Nano-emulsion of chitosan coating containingZCEO (0.5%) and nisin (200 IU/g) were stored at refrigeration temperature and bacterial count were performed on days: 0, 1, 2, 4, 8, 12 and 16. Data were analyzed using repeated measure ANOVA and Bonferroni post hoc tests. Results: Result indicated a significant reduction in E. coli O157:H7 count in all treatments when compared to control group and the highest inhibitory activity was observed in chitosan Nano-emulsion coating containing ZCEO (0.5%) and nisin (200 IU/g). Conclusion: Accordingly, it is suggested that chitosan Nano-emulsion coating with ZCEO and nisinpractically be applied in beef to increase its safety against pathogenic bacteria especially E. coli O157: H7.کلیدواژه ها
Chitosan, Functional coating, Nano technology, Beef, Escherichia coli O157:H7اطلاعات بیشتر در مورد COI
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