N Otadi - Young researcher Club of Islamic Azad University, Science and Research Branch of Tehran- Department of Environmental Engineering and energy
M Shokrzadeh - Department of Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
A G.Ebadi - Department of Experiential sciences, Islamic Azad University of Sari, Sari, Iran

Pesticides are compounds that used by human being, especially farmers for controlling biotical factors for a long time and can bring a lot of environmental problems. Among these compounds chlorine insecticide sprays have high half-life, the property of surviving for a long time and high lipophilicity characteristic. Due to having chronic consequences that resulted from consuming of this compounds, it is necessary to evaluation and analysis in environment especially in foods and also in fishes which have high fat and can carry this kind of insecticide sprays. So we have investigated the remaining of chloride organic insecticide sprays such as D.D.T, D.D.E in the four kinds of fishes in the Caspian Sea. In this investigation we have prepared for different kinds of fishes, which are highly used, available in the Caspian Sea (Sefid - koli- kilca - kefal) that we selected five fish from the four different hunting region (Chalous and Babolsar city and Khazar Abad and Miankaleh region ) in 2004. After cleaning, we prepared Hun muscle of the fished. Then, after the process of extraction, we distilled them in Vacuum by means of organic solvent and at last we evaluated the remaining and reported based on PPM by Gas Chromatography (GC) with ECD detector. The results of insecticides analyzed in four kinds of fishes been showed that the kafal kind of fish in the hunting region of Babolsar had the Highest amount of D.D.T at the rate of 0.031 PPM. Sefid in the hunting region of Babolsar was 0.03 PPM (P<0.05). It is necessary to mention that no research has so far been done to be a criterion for comparison in this area. But, fortunately the amounts of the insecticide sprays has not reached that offered level in fishes, and it raises the alarm.

D.D.T, D.D.E, Organochlorine residues, Caspian Sea, Fish