Investigation of the interaction between DNA and a sample of textile colors by spectroscopic methods

Many of the synthesized compounds in the industry, despite their various applications, have side effects which can lead to diseases in the body. DNA plays a major role in all biological processes. DNA interaction with small molecules such as pigments is important. Some of these compounds can be connected to DNA and impair its conformation and prevent the replication . In this study, in order to provide a clear mechanism for the binding of a chemical color with the physiological macromolecules, the effects of different concentrations of a textile dye, Direct yellow 42, on the calf thymus DNA was investigated using fluorescence, UV-Vis and FT-IR methods. In this study it was determined that DNA has interaction even at very low concentrations of dye; but at high concentrations, sudden changes in the intensity of the absorption spectrum of DNA was observed at the region of 260 nm that shows the denaturation of this biomacromolecule. Calculation of thermodynamic parameters showed that the type of interaction between DNA and Direct yellow 42 is groove and the binding process is spontaneous.The results of this study can provide a new insight into the application of dyes in the industry and the risks associated with their use.

کلیدواژه ها:

DNA ، spectroscopic methods ، thermodynamic parameters ، Direct yellow 42