CaCO3 nanoparticles in combination with multi agent Inhibition of TC1 cell Line
- سال انتشار: 1397
- محل انتشار: سومین کنگره بین المللی پزشکی شخصی ایران
- کد COI اختصاصی: IPMCMED03_043
- زبان مقاله: انگلیسی
- تعداد مشاهده: 572
نویسندگان
Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, IR-Iran
۳Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, IR-Iran
چکیده
Introduction: Cervical carcinoma is the second most common form of cancer and one of the most important causes of death among women around the world. Despite numerous studies, treatment of cervical cancer has remained a considerable problem.The present study Investigation the cytotoxicity effects of CaCO3 nanoparticles in combination with ionizing radiation and hyperthermia on cervical carcinoma (TC1). Methods: In the present study CaCO3 NPs characterization with Transmission electron microscopy (TEM). Then anticancer effects of 150μg/ml CaCO3 NPs, hyperthermia (41 0C for 1 h), irradiation (200 cGy) and 150 μg/ml CaCO3 NPs respectively combined with hyperthermia (41 0C for 1 h) and irradiation (200 cGy) was investigated. Cell viability assays includes MTT, apoptosis, ROS production and detection the levels of Caspase 3, 8 and 9. Also 800 nm Doxorubicin was used as the positive control group. P-values of less than 0.05 were considered statistically significantResults: Results after analysed revealed that 150 µg/ml CaCO3 NPs combination with R+H have a significant (p< 0.01) decrease cell viability (0.133±0.016), a significant (p< 0.05) increase apoptosis percent (74±3.26) of TC1 cells and a significant (p< 0.05) increase generation of ROS (28.52±2.09) in compared with control group. Also, a significant increase (p< 0.05) in the activity of caspase-3 (0.511±0.01) and caspase -9 (0.159±0.02) was observed but caspase-8 activity was not significant (P=0.074) changed.Conclusion: the data obtained suggests that the use the multi-agent CaCO3 NPs combined caused reduced cell viability with increased in generation of ROS of TC1 cells. Also present results showed multi-agent CaCO3 NPs induced cell apoptosis through mitochondrial membrane potential with increase as well as caspase-3 and -9 activations that can be considered in future studies.کلیدواژه ها
CaCO3 Nanoparticles, Hyperthermia, irradiation, Chronic Cervical carcinomaمقالات مرتبط جدید
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