Effect of Berberis vulgaris Root Extract on protoscoleces during hydatid cyst surgery

IntroductionCystic echinococcosis (CE), a zoonotic parasitic infection caused by the metacestode (larvae) stage of dog tapeworm Echinococcus granulosus and recognized as a major economic and public health concern in the world. This study aims to investigate the in vitro scolicidal effect of methanolic extract of Berberis .vulgaris L. roots against protoscoleces of hydatid cysts to use in hydatid cyst surgeryMaterial & MethodsFor this purpose, protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the methanolic extract of the B. vulgaris (0.25-2 mg/ml) were used for 5 to 30 min. .Viability of protoscoleces was confirmed by eosin exclusive testResults In the present study, all of the various concentrations of the B. vulgaris methanolic extract (0.25, 0.5, 1 and 2 mg/ml) revealed significant (P<0.05) scolicidal effects against protoscoleces of E. granulosus in a dose-dependent manner. So that methanolic extract exhibited 100% inhibition against protoscoleces of E. .granulosus at the concentration of 2.0 mg/ml after 10 min incubationConclusion According to the results, B. vulgaris methanolic extract demonstrated high scolicidal activities against : protoscoleces of hydatid cysts in low concentration and short exposure time on in vitro model. However, in vivo efficacy of B. vulgaris also requires to be evaluated using an animal model with hydatid infection

کلیدواژه ها:

Hydatid cyst ، European barberry ، Scolicidal ، Echinococcus granulosus