Voltammetric determination of sulfasalazine by using glassy carbon electrode modified with 2,4-dinitrophenyl hydrazine

Sulfasalazine (salazosulfapyridine,2-hydroxy-5-[(E)-2-{4-[(pyridin-2-yl) sulfamoyl]phenyl} diazen-1-yl]benzoic acid), is a sulfa drug, a derivative of mesalazine (5-ASA),has been made special attention for its therapeutic importance. It used to treat a certain type of bowel disease called ulcerative colitis and Crohn’s disease [1]. In this work, a new method was introduced to modified glassy carbon electrode (GCE) with 2,4-dinitrophenylhydrazine (DNPH) and then, sensitive, inexpensive, rapid and selective voltammetric determination of sulfasalazine (SSZ) performed. Electrode modification, conditions, optimization and electrochemical detection of this molecule was studied by cyclic voltammetry (CV), differential pulse voltammetry(DPV) and chrono-amperometry. The modified electrode presented good linear correlation to SSZ concentration in the range of 1 to 10 μM with low detection limit of 0.35 μM. The proposed method was used for the voltammetric determination of SSZ in human plasma samples.