Identification of some endophytic fungi of Crataegus pentagyna trees from Iran

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 293

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شناسه ملی سند علمی:

BIOCONF20_813

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Endophytic fungi, because of their widespread distribution, biological diversity and production of many valuable bioactive compounds whit antimicrobial, insecticidal, cytotoxic and anticancer activities have been studied. In a natural ecosystem, almost all plants are colonized by fungal endophytes. Endophytic fungi are ubiquitous organisms found in the plants, residing intercellular or intracellular, least for a portion of their lives without causing apparent symptoms of infection. The aim of this study was to identify the endophytic fungi of Crataegus pentagyna tree. Sampling from healthy branches of Crataegus pentagyna trees was done. Isolation of endophytic fungi from plant materials was subjected to Schulz et al (1993) method with slight modification. All of the fungi was consistently identified on the basis of morphological characteristics. In order to molecular identification, the DNA extraction was done by the CTAB containing buffer. The internal transcribed spacer (ITS) regions of ribosomal DNA was amplified using ITS4 and ITS5 primer pairs. The molecular characteristics of the studied fungi were confirmed by morphological characteristics. 7 species as endophytic fungi were isolated and identified including Hypoxylon fragiforme, Biscogniauxia mediterranea, Paraconiothyrium brasiliense, Periconia macrospinosa, Lophiostoma corticola,Paraphoma chrysanthemicola, Lophiotrema sp. neohysterioides . Among the identified fungi Paraconiothyrium brasiliense, Periconia macrospinosa, Lophiostoma corticola, Paraphoma chrysanthemicola, Lophiotrema sp. neohysterioides are as new taxa for mycoflora of Iran. Besides, all identified species are new endophytic records from Crataegus pentagyna trees.

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نویسندگان

Farahnaz Rasouli Miankouh

Department Plant Protection, Faculty of Agricultural Sciences, University of Sari Agricultural Sciences and Natural Resource

Mohammad Ali Tajick Ghanbary

Department Plant Protection, Faculty of Agricultural Sciences, University of Sari Agricultural Sciences and Natural Resource

Heshmatallah Rahimian,

Department Plant Protection, Faculty of Agricultural Sciences, University of Sari Agricultural Sciences and Natural Resource

Valiallah Babaeizad

Department Plant Protection, Faculty of Agricultural Sciences, University of Sari Agricultural Sciences and Natural Resource