Evaluation of genome size of some of Hedera L. taxa (Araliaceae), Emphasizing on Hedera crebrescens species

diverse taxonomic ranks (mainly species) and resolve intricate low-level taxonomies. The correct genome size in Hedera (Araliaceae) has long been debated still unclear. The present study related to this issue and determination of H. crebrescens ploidy level that recently has been identified by M. Bényei-Himmer et M. Höhn. 13 accessions of Hedera measured with flow cytometry (FCM). Fresh leaves of the Hedera samples and (Petroselinum crispum) as a standard reference stained with Propidium Iodide (PI). Flow cytometry measurements showed that the accessions 2CV (3.09 - 6.40 pg). Among 9 diploids examined, the lowest amount of nuclear DNA was 3.09 pg for H. crebrescens while its highest amount of nuclear DNA was 3.22 pg for H. helix which is a statistically insignificant difference. However, among 4 tetraploids a significant difference of 0.64 pg in 2CV recognized for H. hibernica Variegata (5.76 pg) and 6.40 pg for H. hibernica Hamilton . According to this study, new taxa H. crebrescens would be diploid, previously these taxa considered to be H. hibernica. Ploidy levels checked by counting the chromosome number. The results of this study confirm the separation of this newspecies from H. helix and H. hibernica species

کلیدواژه ها:

Propidium Iodide (PI) ، Ploidy level ، Flow cytometry ، nuclear DNA ، 2CV