Effect of iron sulfate foliar spray on enzymatic and non-enzymatic defense of Dracocephalum moldavica L. under salinity stress

An experiment which was conducted in greenhouse aimed to investigate the effects of iron sulfate, as a salinity stress modifier, on antioxidants, and the percentage of radical inhibition of Diphenyl 2-picryl hydrazine-DPPH in Dracocephalum moldavica L. The plants in the 8th leaf stage were treated with different concentrations of Sodium Chloride (0, 50, 100 mM) alone, and also along with different concentrations of Fe2(SO4)3 (0, 30, 60, and 90 PPM). The results showed that due to salinity stress, totalphenol content, Flavonoid, DPPH radical inhibitory percent, and the activity of guaiacol peroxidase, ascorbate peroxidase, catalase and glutathione reductase enzymes were increased in shoot and root in 100 Mm NaC1; using iron sulfate in different levels of salinity lead to increased enzyme activities compared to control plants and salinity treatment without iron fertilizer. By increasing the concentration of sodium chloride, the activity of enzymatic antioxidants was increased. The above mentioned enzymes‟ activities in plants which were under simultaneous treatment of salinity and iron sulfate had significant increase comparing to control plants. The results revealed that using iron sulfate improves antioxidant defense of Dracocephalum moldavica L. under salinity stress

کلیدواژه ها:

Salinity ، Iron Sulfate ، Antioxidant enzymes ، Dracocephalum moldavica L