Induced changes in phenylpropanoid compounds of Linum album hairy roots by chitosan

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 505

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شناسه ملی سند علمی:

BIOCONF20_738

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Linum album is a medicinal plant of Linaceae family that contains some lignans such as, podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). Lignans have anti-viral and anti-carcinogenic properties. Effect of various biotic elicitors has been studied on phenylpropanoid biosynthetic pathway in this species. In this study, the response of the phenylpropanoid biosynthetic pathway to chitosan as a biotic elicitor in Linum album hairy root was investigated. The 10 days old hairy roots were elicited by 200 mg L-1 low molecular weight chitosan. The amount of lignans, total phenolic acids, flavonoids, and flavonols and activity of phenylalanine ammonia-lyase (PAL) and tyrosineammonia-lyase (TAL) as important biosynthetic enzymes in this pathway, were determined at 24, 48 and 72 h after treatment by HPLC and spectrophotometric techniques. The podophyllotoxin and 6-methoxy podophyllotoxin content increased during elicitation while there was a significant decrease in lariciresinol. The chitosan treatment enhanced the accumulation of total phenolic acids and flavonols but reduced the total flavonoids. Simultaneously, the PAL and TAL activity increased at 24 and 48 h after treatment. These results provide an evidence regarding the elicitor role of chitosan on the phenylpropanoid biosynthetic pathway. Different branches of this pathway show various responses. This polysaccharide up-regulates the production of PTOX and MPTOX, phenolic acids, and flavonols by affecting PAL and TAL activity. Lariciresinol decrease can be due to consumption of this molecule as a PTOX and MPTOX precursor. These findings suggest that depending on plant needs, flavonoids precursors shift to different biosynthetic pathways concomitant with total flavonoid reduction.

نویسندگان

Elaheh Samari

Plant Biology Department, Faculty of Biological Science, University of Tarbiat Modares, Tehran, Iran.

Mohsen Sharifi

Plant Biology Department, Faculty of Biological Science, University of Tarbiat Modares, Tehran, Iran.

Faezeh Ghanati

Plant Biology Department, Faculty of Biological Science, University of Tarbiat Modares, Tehran, Iran.

Najmeh Ahmadian Chashmi

Biology Department, Faculty of Science, University of Mazandaran, Mazandaran, Iran.