Genetic diversity of Brachypodium distachyon (Poaceae) in Iran based on microsatellite markers

The Brachypodium distachyon complex comprising three annuals; B. distachyon. B. stacei and B.hybridum is a model for polyploid grasses and temperate crop evolution. These species are ephemeral, inbreeding, widely distributed and energy-crops in their native distribution in circum-Mediterranean. Several studies have been performed on the subject of genetic diversity of this model plant, including using SSRs to study genetic diversity of B.distachyon populations in Turkey, genetic diversity of B.hybridum populations using SSRs, evaluating 90000 SNPs for confirming the hybrid nature of B.hybridum, and studying 9 populations of tetraploid B.hybridum in Tunes using 8 indels. New sequences in this species showed that there are hundreds of new polymorphisms not already seen in reference sequences. In this study, genetic diversity and structure of 72 individuals from natural populations of this species in Iran were studied using 20 SSRs and ISSRs. PCR products separated by PAGE and showed that all markers were polymorphic. Our confirming results showed that there were no relations between groupings of populations and their geographic origin or elevation and that long-distance dispersal was a major factor shaping the genetic structure of the populations. Observations were comparable to those studied in Tunes

کلیدواژه ها:

Brachypodium ، Microsatellite ، Genetic diversity ، Iran