Biopriming of lettuce seeds (Lactuca sativa L.) with Trichoderma fungi and its mutants and study of germination and some of its morphological characteristics

Increase the germination and growth indices using methods such as biopriming is important. To this end, an experiment was conducted to study the effect of germination with five species of native Trichoderma fungus of Iran on some of the vegetative traits of lettuce seeds seedlings. The effect of induced-mutation by gamma irradiation on the Trichoderma genome and probably increase in the efficiency of biopriming via using mutated Trichoderma isolates was studied. Experiments with three treatments: control, inoculated with wild-type Trichoderma based bio-formulation and inoculation with mutants Trichoderma, at the Nuclear Agriculture Research Institute in the form of a completely randomized design with three replications was done. Evaluation of traits (germination percentage, stem and root length, fresh and dry weight) were evaluated using standard ISTA method. Results showed the highest germination percentage was in seeds coated with Trichoderma. Due to the use of fresh lettuce, fresh weight of root and especially shoot, fungal treatments showed significant improvement. The important index of stem dry weight was better in fungal treatments than control. But the length of the seedlings from the coated seeds was less than the control. By comparing these two indicators, it can be said that Trichoderma by providing the food needed for the plant, avoid wasting plant energy to increase its length. The amount of root hairs in the roots of buds which had Trichoderma were much more control. Generally, due to the use of lettuce, it is recommended to use Trichoderma biologic fungi to enhance the performance of lettuce

کلیدواژه ها:

Biopriming ، Lettuce ، Germination ، Trichoderma