Assessment of the antimicrobial effect of Cistanche sp. on the planktonic forms and biofilm structures of pathogen bacteria

Nowadays, the resistance of microorganisms to various antibiotics is a serious concern. Also, study on herbal plants is very important. In this study the antimicrobial effect of the juice of Cistanche sp was investigated on planktonic and biofilm forms against some pathogens. Antimicrobial effect of Cistanche sp was determined by two methods, well and disk diffusion. Seven strains of standard bacteria, 4 strains of clinical bacteria and 3 strains of fungi were used. Minimum inhibitory concentration and Minimum lethal concentration (0.2-50 mg/ml) were determined by broth macrodilution. Antibiofilm effects, biofilm formation and biofilm degradation, were studied by microtiter plate in 5, 10, 15 and 20 mg/ml. According to the results, the disk diffusion method was better than well method. The average of inhibition zone in 36.4% of bacteria was ≥ 10 mm, whereas it was ≤ 9mm in 9.09 of bacteria. The juices of Cistanche sp in 5 mg/ml could stop biofilm formation in 63.64% of bacteria. The most stop biofilm formation was in Escherichia coli PTCC1330 and Micrococcus luteus PTCC1110 (> 80%). The greatest eradication of biofilm structures was observed in Micrococcus luteus PTCC1110 (58.75%) and Pseudomonas aeruginosa (58%). In this study was investigated that the juice of Cistanch sp has not only antibacterial but also anti-biofilm effects.

کلیدواژه ها:

Cistanche sp ، Herbal Plants ، Antimicrobial ، Pathogen Bacteria ، Biofilm