Evaluation of the effects of silver nanoparticles and alcoholic extract of Achillea wilhelmsii on pathogenic bacteria Staphylococcus aureus, Bacillus cereus, Escherichia coli andPseudomonasaeruginosa

In recent years, the use of medicinal plants has increased in the treatment of infections rather than antimicrobial drugs. Nanotechnology also plays a major role in the medical arena, which uses nanoparticles like silver to fight microbial resistance. The aim of this study was to compare the effects of silver nanoparticles and alcoholic extract of Achillea wilhelmsii on some pathogenic bacteria and also silver nanoparticles.The plant was examined from the natural areas of Marand city in East Azarbaijan province and was identified as Achillea wilhelmsii according to herbarium characteristics in Islamic Azad University of Marand district. The alcoholic extract of this plant was prepared and the effect of concentrations of 50 mg / ml, 100 mg/ml, 200 mg/ml, 400 mg / ml of this extract and concentrations of 10 mg / ml, 20 mg / ml, 40 mg / ml, 80 mg / ml of silver nanoparticles by Well Diffusion Method on S. aureus, B. cereus, E. coli and P. aeruginosa. Minimum Inhibitory Concentration and Minimum Bactericidal Concentration (MIC/MBC) was determined on bacteria by a dilution method.The results showed that the inhibition effect of Achillea wilhelmsii on grampositive bacteria is more than gram-negative bacteria while the inhibitory effect of silver nanoparticles on gramnegative bacteria is more than gram-positive bacteria. The effect of the combination of Achillea wilhelmsii and silver nanoparticles was much greater than the effect of each of them. The composition showed the highest effect on P. aeruginosa and the lowest sensitivity to S. aureus. The results of this study showed that the alcoholic extract of Achillea wilhelmsii is considered to be an antibacterial properties Medicinal Plants group that can be considered as an adjunct to further studies for use in vivo conditions and replacement with common chemical agents in the treatment of infections.

کلیدواژه ها:

Medicinal plants ، Silver nanoparticles ، Achillea wilhelmsii