Effect of Bifidobacterium strains isolated from baby feces on Acinetobacter biofilm

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 299

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شناسه ملی سند علمی:

BIOCONF20_530

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Probiotics are beneficial organisms that have their therapeutic effects by replacing the microbial flora.Acinetobacter is one of the most important bacteria that causes the infection of the hospital and has high resistance to antimicrobial agents. The probiotic Bifidobacterium has a significant effect on Acinetobacter biofilms and can beconsidered as an alternative antibiotic product. In the laboratory, 100 samples of skin scars and urine twelve strains of Acinetobacter were confirmed by standard biochemical tests and for biofilm formation, they were selected on a polystyrene plate. Biofilm formation was confirmed through ELISA Reader. The stools of six infants were cultured in MRS Broth (Man-Rogasa-Sharpe) medium. After isolation and identification,Bifidobacterium strains were added to biofilm in different dilutions. According to the average results of both series of repeat tests, 75% of Acinetobacter strains were affected by bifidobacterium and 62.5% of the strains were influenced by the antibiotic ciprofloxacin. The probiotic Bifidobacterium has a significant effect on Acinetobacter biofilms and can be considered as an alternative antibiotic product.

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نویسندگان

Masoumeh Moradi Moghadami

Department microbiology, Faculty of Basic Sciences, University of Islamic Azad University of Tehran North Branch, Iran

Farzaneh Hosseini

Department microbiology, Faculty of Basic Sciences, University of Islamic Azad University of Tehran North Branch, Iran