Assessment of new antibiotics application for controlling of bacterial canker of stone fruits in laboratory conditions

Bacterial canker caused by Pseudomonas syringae pv.syringae is one of the most important bacterial diseases of plants that can causes canker, leaf spot and die-back in stone fruit trees and considerable yield losses in these trees. During the study years, 2015-2016 in different areas of the Khoy (Firooragh- Rahal- Boozghoosh) were surveyed and some suspected plant samples with canker symptom were collected.Based on biochemical tests the cause of bacterial canker of stone fruits primarily in the area was identified. Molecular identification of selected isolates, polymerase chain reaction was performed by the specific primer. PCR with primers B1 and B2 test results pathovars Pseudomonassyringaepv. syringae (Pss), fragment 752 bp from the 3 isolates were amplified view. Then, the effect of 10 new antibiotics, including Ciprofloxacin, Clindamycin, Co-amoxiclav, Cefalexin, Azithromycin, Erythromycin, Ceftizoxime, Cefazolin, Tetracycline on the population of bacterial isolates of Pss were evaluated in vitro. Based on the results, all selected antibiotics can decrease the bacterial population but there are significant differences in their control capability. Based on the results, Cefalexin and Erythromycin have the highest and lowest impact on bacterial growth respectively.

کلیدواژه ها:

Stone fruit trees ، Bacterial canker ، Antibiotics ، Pseudomonas syringae pv. Syringae