Evaluation of Hsa-miR-940 expression in tumoral and marginal tissues of the patients with breast cancer

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 365

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شناسه ملی سند علمی:

BIOCONF20_482

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Breast cancer is one of the most common cancers and the second cause of cancer-related death among women. During the recent years, microRNA (miRNA) has become increasingly recognized as an important regulator of cancer cell biology. miRNAs are small non-coding RNAs with 18–22 nucleotides that bind the 3′UTR of targetmRNAs to reduce their stability and/or translation. The expression of Has-miR-940 has been shown to play an important role in various cancers. The present study aimed at evaluation of Hsa-miR-940 expression in breast cancer. The tumoral and marginal tissues of the breast cancer patients collected and stored at -80 C until use. Total RNAwas isolated from the breast cancer tissue using RNX-Plus according to the manufacturer’s protocol. Then cDNA was synthesized by PrimeScriptTM RT reagent Kit (TaKaRa). Has-miR-940 expression was analyzed by real-time PCR. U6 was used as an internal control gene. Primary data from Real-time PCR indicated downregulation of HasmiR-940 in tumoral tissues in comparison with the marginal tissues.

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نویسندگان

Ali Abedinzadeh Geshlagi

Department of Cellular and Molecular Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran,Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran

Mohammad Khalaj-Kondori

Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran

Raheleh Majdani

Department of Cellular and Molecular Biology, Faculty of Basic Science, University of Maragheh, Maragheh, Iran.

Mohammad Ali Hosseinpour Feizi

Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran