The study of IDOL gene expression changes in adipose tissue of male rats subjected to chronic immobilization stress

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 327

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

BIOCONF20_478

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Immobilization stress is effective on physiological systems. So that it increases, the risk of cardiovascular disorders and disturbs cholesterol and increases the level of LDL in the blood. IDOL is a protein that regulates cholesterol and LDL levels by decomposing LDL receptors. IDOL reduces the absorption of LDL from the blood and increases its serum levels. The aim of this study is to determine the changes in IDOL gene expression in adipose tissue of rats exposed to chronic immobilization stress. 10 male Wistar rats were divided into groups of Control and chronic immobilization stress. Rats group of chronic immobilization stress were exposed to 6 hours for 21 consecutive days. At the end of this period, animal adipose tissue was removed. Further, RNA extraction and cDNA synthesis were performed and changes in the expression level of IDOL gene in this tissue were evaluated quantitatively by Real-Time PCR. Based on the results, immobility stress reduced the expression of IDOL gene in adipose tissue of rats. Because IDOL gene expression has been reduced due to immobility stress, cholesterol and LDL cholesterol levels are likely to be lower in this group. The results show that the effects of immobility stress are not similar to low-mobility complications.

نویسندگان

Raana Hasanlo

Department of Genetic, Zanjan Branch, Islamic Azad University, Zanjan, Iran

Sanaz Mahmazi

Department of Genetic, Zanjan Branch, Islamic Azad University, Zanjan, Iran

Mahdi Rahnama

Department of Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran