The Effect of hydroalcoholic extract of spinach on leptin gene expression changes in adipose and muscle tissues of male rats subjected to chronic immobilization stress

Leptin gene regulates stability, energy, and metabolism, and it is most important function is to reduce body weight by reducing appetite and reducing feed intake and increasing energy production from body fat stores. Stress disrupts the function and physiology of the body and changes the secretion of related hormones in different tissues. Spinach has beneficial properties, including cholesterol reduction and anti-anxiety, and is used to treat many diseases. The present study examined the effect of spinach extract on leptin gene expression changes in Adiposeand Muscle of rats under chronic immobilization stress. 30 male Wistar rats were divided into 6 groups: 1) Control group. 2 and 3) Spinach extract treatment group with 200 mg/kg and 400 mg/kg. 4) chronic immobilization stress group. 5 and 6) chronic immobilization stress group with spinach extract at 200 mg/kg and 400 mg/kg. Group 2, 3, 5 and 6 mice received hydroalcoholic extract of spinach for 21 consecutive days. Mice Group 4, 5 and 6 of 6 hours for 21 consecutive days were bound. After the Adipose and Muscle tissue separation, RNA extraction and cDNA synthesis were performed and the changes in leptin gene expression in these tissues were quantitated using Real-Time PCR. Data analysis showed that the level of leptin gene expression in adipose tissue was significantly increased by chronic immobilization stress. But the spinach extract reduces this change of expression. In muscle tissue, leptin expression is reduced by immobility stress and spinach extract increases its rate. Leptin, which secretes from the adipose tissue, is a controlling factor in metabolism. The male spinach extract has moderated its expression level. Therefore, it may be possible to spinach as a substance to prevent the effects of stress.

کلیدواژه ها:

Chronic immobile stress ، Spinach extract ، Leptin gene