In silico analysis of immune system stimulation by asparaginase enzymes produced by bacterial endophytes

Unlike normal cells, cancer cells receive asparagine amino acids from extracellular sources. In order to evacuate this amino acid from the surrounding of cancerous cells, some cancer patients receive the commercially available asparaginase enzyme producing by Escherichia coli as a medicine. Due to the immunogenicity of this enzyme, allergic reactions and immune responses can be observed in the patient s body. In recent years, regardless of immunological characteristics, many studies have been conducted on the production of this enzyme by endophyte bacteria. In the present study, the possibility of production of L-asparaginase with more appropriate immunological parametersby this kind of bacteria was investigated through bioinformatics methods. To investigate the presence of this enzyme in endophytic bacteria BLAST-Pwith E.coli asparaginase enzyme sequence against 275 endophytic bacteria were performed. The results with identity more than 35%, coverage of more than 50%, and E-value less than 4-10 were selected. To prediction of allergenicity, antigenicity and the number B and T lymphocytes epitopes, E.coli asparaginase enzyme sequence and selected sequences were analyzed with Algpred, Antigenic Peptide Prediction, VaxiJen, ABCpred, ProPredI and ProPred software. According to the results, a total of 9 sequences of known and hypothetical proteins were identified in 6 bacteria. The comparison of thementioned immunological characteristics of these sequences showed that some of the asparaginase enzymes produced by endophytic bacteria possess more suitable immunological indices. Therefore, the therapeutic use of these enzymes is possible.

کلیدواژه ها:

Asparaginase ، Escherichia coli ، Endophytic bacteria ، Bioinformatics software