Effect of silica-chitosan nanocomposite encapsulated epigallocatechin gallate on SKOV3

Ovarian cancer is one of the most common gynecological cancer among women. It has an inactive and asymptomatic course which led to the lately diagnosis in the advanced stages that cause cancer cells became resistant to the most of the drugs. Epigallocatechin gallate is one of the polyphenols which is derived from flavonol and have antioxidative, antiproliferative and pro-apoptotic effect against ovarian cancer cell lines. Chitosan is one of the most attractive natural polysaccharides because of its special characteristics such as biodistribution, biodegradation, and low toxicity. In this study, Chitosansilica nanocomposites were synthesized through the sol-gel method in the presence of APTES and glutaraldehyde as a linker. Then, their absorbance and particle size were characterized by Fourier transforms infrared (FTIR) spectroscopy and TEM. Then, EGCG encapsulated in the nanocomposite. SKOV3 cell lines were treated with free and encapsulated EGCG. FTIR spectroscopy approved the process of nanoparticle synthesis. Size of nanoparticles was between 75-150nm. The survival rate of ovarian cancer cell lines which treated with EGCG alone and encapsulated EGCG was evaluated by MTT assay. Encapsulated EGCG could decrease the survival rate in SKOV3 cell line through a dose and time-dependent manner and also it has less cell viability in contrast with cells which treated with EGCG alone.

کلیدواژه ها:

Epigallocatechin gallate ، Silica ، Chitosan ، SKOV3