Ancient DNA extraction, identification, molecular cloning and enzymatic enhancement

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 481

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شناسه ملی سند علمی:

BIOCONF20_440

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

In the field of scientific research, it is anticipated that the twentyfirst century, contrary to the twentieth century, was dominated by physics, a century of biology. Genetic information derived from the DNA of human bones can help to study the relationships existing between ethnic groups and in smaller dimensions of groups and individuals buried in a cemetery, and can be useful in determining the sex of skeletons. In the study of paleopathology on bone samples, it is possible to identify inherited diseases and infectious diseases that have contaminated the DNA in the bones. The DNA obtained from archeological and paleontological remains allows for the timely return and study of the genetic linkages of extinct organisms to their contemporary family. This poses a new perspective on the evolution of organisms and their DNA sequencing. However, there are many technical problems in this field and they require precise criteria to ensure the credibility of the findings, especially in the study of human remains. In recent years, the study of aDNA has expanded and attracted many enthusiasts. The study of ancient DNA helps to clarify the historical events from a more comprehensive viewpoint and can be used in various areas of research and research. At present, it is necessary to use biotechnology in the development of cultural heritage, because its use in understanding the culture of the Iranian ethnic groups can reveal some dark points in Iranian history. One of its major achievements is the acquisition of necessary information for the development and analysis of genetic changes that have been made among Iranian ethnic groups during the history of immigration, wars, and so on. It should be noted that studies in the field of ancientgenetics require specialized laboratories with their own standards. The method used in ancient DNA studies is that; the specimen is taken from the tooth structure, femur, pterosa, tibia and humerus, and genetic studies are done in the Dloop region of the mitochondria section. The sample conditions are performed according to the skeleton conditions obtained from the ancient area of the mentioned sections. In addition, the person responsible for this should be prominent in its entirety, in order not to create an error during the operation. The conditions of work are carried out in completely specialized laboratories. To remove any bone marrow, separate gloves should be used to prevent any transmission of contamination from person to sample. Also, the environmental conditions of the laboratory are such that no other genetic and molecular studies in this environment are accepted. The cultural heritage research institute, as the representative of the Institute for theConservation and Restoration of Historical-Cultural Works, has taken steps in this field over the past two years with the assistance of reputable scientific centers such as the Human Genetic Research Center Baqiyatallah Azam (AS).

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نویسندگان

Parastoo Erfanmanesh

Research Institute of Cultural Heritage and Tourism, Iran

Kamran Ahmadi

Research Institute of Cultural Heritage and Tourism, Iran