Study of the interaction between indinavir and complex (DNA-H1) by multispectroscopic techniques

The interaction of small molecules with DNA plays an important role in many biological processes .DNA binding studies have remarkable relevance and implications in drug design aspects and cancer chemotherapy. Histones act as spools around which DNA winds. This enables the compaction necessary to fit the large genomes of eukaryotes inside cell nuclei .Histones undergo posttranslational modifications that alter their interaction with DNA and nuclear proteins. Indinavir group of HIV drugs called protease inhibitors. The enzyme in HIV called protease is blocked by PI. Pls, prevent HIV from multiplying and can reduce the amount of HIV in the body. The chemical formula of Indinavir is CH36H47N504, and its molecular weight is 613.79. The interaction between complex (DNA-H1) and indinavir has been studied by using different spectroscopic techniques vis., resonance light scattering (RLS) spectra and circular dichroism (CD) spectra, in physiological buffer pH=6.8. The RLS method determined the critical aggregation concentration of drug on complex (DNA-H1). When indinavir was added to (DNA-H1) complex, the RLS intensity of the system was increased. It is known that RLS enhancement could be the result of the enlargement of the molecular volume and large size of the complex Circular dichroism is a suitable method for monitoring thesecondary and tertiary structure of proteins. Quantitative analyses of the CD spectra of the interaction between (DNA-H1) and drugs secondary structure. Change of thesecondary structure of DNA-H1 involving an increased of α-helix, and chirality has increased Electrostatic forces and hydrogen bonds.

کلیدواژه ها:

Indinavir ، Complex-DNA-H1 ، RLS ، CD