The acetylcholinesterase inhibitory activity and antioxidant properties of methanol extract of different organs of Euphorbia macrocladaBioss and Glaucium grandiflorumBioss and Huet

The oldest hypothesis for Alzheimer s disease, which many available drugs have been made upon it, is the cholinergic hypothesis. This hypothesis suggests that Alzheimer s disease results from the reduction of acetylcholine. One of the main reasons for acetylcholine reduction is the high activity of acetylcholinesterase, so inhibition of this enzyme can contribute to the control of the disease. Since the effect of methanolic extracts of Euphorbia macroclada Bioss and Glaucium grandiflorum Bioss and Huet has already been demonstrated, the purpose of this study is to investigate and identify organs of the above plants that show maximum inhibitory activity, as well as the measurement of the antioxidant activity of these organs. Methanol extracts of isolated organs of the plants were prepared using a rotary evaporator. The inhibitory effects of the extracts were investigated in six concentrations at 405 nm wavelength using a microplate reader. Antioxidant activity of the extracts was measured using the DPPHscavenging test. The highest inhibitory activity of Glaucium grandiflorum was observed at the 8 mg/ml concentration (100% inhibition and IC50= 0.01mg/ml). The most inhibitory activity of Euphorbia macrocladawas related to an 8 mg/ml concentration (100% inhibition and IC50= 1/12 mg/ml). Glaucium grandiflorum organs have antioxidant activity of 100% and EC50 = 0/36mg/ml, EC50 = 0/2mg/ml for flower and leaf respectively. Euphorbium flower and leaves also have 100% antioxidant activity and EC50=0/035 mg/ ml EC50= 0/032 mg/ml respectively. The results of this study indicate that methanol extract of Glaucium grandiflorum and Euphorbium flower and leaf organs have a significant inhibitory effect on acetylcholine aesthetic activity. Therefore, it may be possible in feature studies of these extracts to find potential sources of new drugs for the treatment of acetylcholinesterase related diseases.

کلیدواژه ها:

Acetylcholinesterase ، Inhibitor ، Methanol extract ، Euphorbia macroclada ، Glaucium grandiflorum