Isolation of immunodominant proteins of Naja Naja (oxiana) snake venom

Snake venom is a complex mixture of proteins, peptides, enzymes, carbohydrate, and mineral. Snake venom contains a variety of chemicals including pharmacological and toxicological properties. The innate immune system is the first line of defense against toxin and microbe. The aim of the present study is to investigate and isolate immunodominant proteins of Naja oxiana snake venom. Identification was performed by SDS-PAGE 15% and western blot analysis. Subsequently, four sharp protein bonds 14, 22, 32, 64 kDa, were appeared in nitrocellulose paper. The next step identified proteins were isolated directly by Electro-elution from preparative gel electrophoresis. To the best our knowledge, these proteins may be a candidate for specific antivenom or antiserum against Naja oxiana

کلیدواژه ها:

Naja Naja ، Snake venom ، Immunodominant protein ، Electro-elution