Isolation of multiple drug-resistant genes on exfoliative toxin B-encoding plasmid in Staphylococcus aureus obtained from skin infection samples

The exfoliativetoxin is Staphylococcus aureus various factorsthat can cause colonization and virulence.The aim of this study was to investigate the presence of several antibiotic-resistant genes on exfoliative B-encoding plasmids in S. aureus-infected skin specimens. 100 wound samples collected from Tehran Hospitals. Bacteria were identified by standard biochemical and microbiological tests. Antibiogram test was performed for Staphylococcus aureus isolates. DNA was extracted fromresistanceStaphylococcus aureus. Presence of antibiotic-resistantetb, aac, and msrA genes was identified by PCR. From 100 samples, 60 samples were Staphylococcus aureus. Resistance to erythromycin, gentamicin, tobramycin, ciprofloxacin and linezolid was 3 (5%), 1 (2%), 1 (2%), 1 (2%) and 0 (0%) respectively. Frequency of msrA, aac and etb genes were 29 (48.3%), 45 (75%) and 37 (61.7%) respectively. Frequency for simultaneously presence of the etb / aac, etb / msrA, msrA / aac and msrA / aac / etb gene were 28 (46.6%), 22 (36.6%), 22 (36.6%) and 18 (30%) strains. In this study, the etb gene was indicated in most strains. As also, investigation of virulence genes in Staphylococcus aureus skin infection samples showed frequency of resistance pathogen genes were increased

کلیدواژه ها:

Staphylococcusaureus ، Resistance genes ، Exfoliative toxin ، Plasmid ، Skin infection