The G-quadruplex structure of COX-2 gene promoter: a novel structure to inhibit the COX-2

Overexpression of COX-2 gene, an inducible inflammatory gene, has a special role to begin the tumorigenesis and angiogenesis and to increase of colorectal tumor polyps. The other studies have shown the potential of G-quadruplex structure formation of oncogenes promoter. So the formation of a G-quadruplex structure on promoter region could be used as a strategy to control cancer. One region of a COX-2 promoter which has the G-quadruplex formation potential has been identified by QGRS online software. The formation and stability of the G-quadruplex structure of this sequence have been assayed in the condition of 0-2 μM of Gquadruplex stabilizer vs of control negative by PCR Stop method which assayed the intensity of bands on PAGE 15%. The potential sequence which has been founded by QGRS is a 29bp sequence and its G-score is 32 which show this sequence has the potential to form a Gquadruplex structure. The results have shown that the intensity of PCR product bands has been decreased by increasing the TMPyP4 concentration especially 1 and 2μM vs the control sample which is without TMPyP4. But there were not any decreasing of PCR product intensity in control negative sequence by increasing of TMPyP4 vs of the control sample. Based on this study, there is a change structure has been formed in this sequence after its treatment by G-quadruplex stabilizer compare to the control negative sequence. So this changing is probably because of the G-quadruplex formation

کلیدواژه ها:

G-quadruplex ، Prostaglandin-Endoperoxidase-2 ، Downregulation ، PCR stop assay