The interaction effects of ZnO nanoparticle application and salinity stress in two populations of Trigonella plants

Fenugreek (Trigonella foenum-graecum L.) is a member of the family Fabaceae. It is an important crop cultivated in semi–arid regions of the world. Salinity is one of the most important abiotic factors limiting plant growth and yield. The purpose of this study was to investigate the effect of ZnO nanoparticle and salt stress on vegetative growth of two populations of trigonella plant. In 4 leafy stages, the interactive effects of ZnO nanoparticle solution (0, 1000 and 3000 mg/L) and salinity stress (0, 75, 150 and 225mMNaCl) were studied. The effects of ZnO nanoparticle and salinity stress were investigated on some biochemical parameters such as lipid peroxidation, proline, protein and nonredundant carbohydrates content. Data were analyzed with Minitab. Specifically, increased salt concentration caused an increase in proline and reductant sugars contents in two populations, while the same factor caused an increase in MDA in mashhadian population. Interaction effects of 3000 mg/l ZnO and 225 mM NaCl caused the increase of proline and reductant sugars in Ardestanian population and the reduction of two mentioned parameters in Mashadian population. 225mM NaCl enhanced Car content and decreased Chlb content in Ardestanian population. ZnO nanoparticles improved these adverse effects. 3000 mg/l ZnO nanoparticle ameliorated reduction in protein caused by high salinity. We suggest that The effect of ZnO nanoparticles on reducing the effects of salinity stress depends on the concentration of nanoparticles used, as well as the level of resistance of Fenugreek populations under salinity stress.

کلیدواژه ها:

Trigonella ، Salinity stress ، ZnO nanoparticle