Effect of biologically synthesized silver nanoparticles on the phenol and flavonoid parameters of Rosmarinus officinalis L.

Today, limited information is available about nanomaterials effects on human health, in spite of its widespread use. Since the particles in nanoscale have new properties, the silver particles exhibit significant physicochemical properties and biologically activity because of small size properties. These distinctive characteristics make silver nanoparticles more practical in medical programs including antibacterial, antifungal, antiviral, anti-inflammatory and destroying cancer cells. Also, the silver nanoparticles have been more considered by researchers due to their high surface to volume ratio. Rosmarinus officinalis L. belongs to a lamiaceae family, has antibacterial and anticancer properties. This plant has anti-oxidant properties, because of phenolic diterpenes in their leaves extraction. Polyphenols are one of the largest natural anti-oxidant in plant kingdoms. Usage of polyphenols in plant led to decrease of disease like cancer. Rosmarinus officinalis L. plant, because of polyphenols such as carnosic acid has maximum anti-oxidant activity. This experiment was arranged in a factorial based on a completely randomized design with three replications in a research greenhouse of Sana Institute of Higher Education. Purchased seedlings from the agricultural greenhouse of Karaj after planting in soil and assuring from the successful establishment in a pot and the emergence of 4 to 6 leaves of the plant, treated with biologically synthesized silver nanoparticles (prepared using sage ethanolic extraction and silver nitrate) (40 mM) applied on the plant by spray solution. Pods containing sage transported to a greenhouse with 16 hours of light (8000 to 10000 Lux light) and 8 hours of darkness, relative humidity of 60% and an average temperature of 5 ± 25°C. The nutritional requirements of the plant were provided by a Hoagland nutrition solution. Total phenolic and flavonoid compounds were measured after performing the above steps. The results illustrated that the plant samples treated with synthesized-nanoparticles had 78% phenol and 82%flavonoids more than control samples. From the above results, it can be concluded that synthesized nanoparticles by plant extract can act as an elicitor in increasing secondary metabolites and subsequently can be used to produce more of these important compounds with medicinal attributes followed by a new pathway will be identified to control diseases.

کلیدواژه ها:

Rosmarinus officinalis L. ، Biologically synthesized silver nanoparticles ، Phenol ، Flavonoid