Increasing oil recovery using Bacillus halodurans OT24 isolated from oily clarifier

Considering the growth of global demand for oil and gas, the use of new technologies for extracting in situ oil of reservoirs with enhanced oil recovery processes (EOR) is necessary. Microbial enhanced oil recovery (MEOR), by the use of microorganisms and their biological products, improve the extraction of residual oil of reservoirs and is more cost effective and less harmful to the environment. The purpose of this study is to investigate efficient microorganisms in MEOR. In this study, the oil contaminated samples were collected from clarifiers in Tabriz petroleum refinery and the effective bacteria were isolated. The syringe model was used for MEOR studies. Experiments with bacterial inoculation amounts of 0.1, 0.2, 0.5 and 1 mL at 47 c by different heating times were carried out. After giving the system an adequate time, 5% brine solution was used to recover the oil from syringes. In the end, according to the results, the oil recovery factor calculated. Based on molecular and biochemical methods Bacillus Halodurans OT24 was identified and its efficiency in the MEOR process was investigated. The highest calculated recovery factor was 90% in 1mL of inoculation suspension and 24 hours of heating time. According to the results of the study, the most important factors causing the recovery factor for this bacterium were acid production and a high amount of biomass

کلیدواژه ها:

Microbial enhances oil recovery ، Bacillus halodurans ، Oily clarifiers