Optimization of effective parameters on biosurfactant production by Bacillus licheniformis OT9 using response surface methodology
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 294
نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
BIOCONF20_301
تاریخ نمایه سازی: 28 اردیبهشت 1398
چکیده مقاله:
Biosurfactants are various amphipathic structures produced by different microorganisms. Amphipathic structures of these materials lead to a reduction in surface tension, interfacial tension, and solubility of immiscible components like water and oil. One of the main applications of biosurfactants is their usage in microbial enhanced oil recovery (MEOR) processes. The aim of this study is the optimization of growth medium and physicochemical parameters effective on biosurfactant production in this isolate. Carbon and nitrogen sources, crude oil, NaCl concentration, temperature and time were investigated as the effective parameters on biosurfactant production by Bacillus licheniformis OT9 using experiment design (minitab.v16). The produced biosurfactant amount in each experiment was measured by the surface tension reduction (tensiometry). First, molasses and yeast extract were chosen qualitatively as the carbon and nitrogen sources. Then, using the Plackett–Burman design, 4 factors (nitrogen, temperature, time and crude oil) were determined as effective among the 6 factors mentioned above. After that, optimization of the effective parameters was carried out using Central Composite Design (CCD). The statistical method presented a quadratic equation as a model with the regression value of 94.13%. The optimum values were determined to be: yeast extract 1.12 gr⁄l , crude oil 1%, time 24 hours and temperature 55 c. These optimum values resulted in the maximum value of produced biosurfactant and surface tension reduction down to 39.5 mN⁄m .
کلیدواژه ها:
نویسندگان
Mahsa Golghasemi Sorkhabi
Faculty of Petroleum and Chemical Engineering, University of Tabriz
Hassan Aghdasinia
Faculty of Petroleum and Chemical Engineering, University of Tabriz
Gholamreza Zarrini
Laboratory of Microbiology, Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz
Elnaz Ghasedi
Laboratory of Microbiology, Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz