Optimization of effective parameters on biosurfactant production by Bacillus licheniformis OT9 using response surface methodology

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 291

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شناسه ملی سند علمی:

BIOCONF20_301

تاریخ نمایه سازی: 28 اردیبهشت 1398

چکیده مقاله:

Biosurfactants are various amphipathic structures produced by different microorganisms. Amphipathic structures of these materials lead to a reduction in surface tension, interfacial tension, and solubility of immiscible components like water and oil. One of the main applications of biosurfactants is their usage in microbial enhanced oil recovery (MEOR) processes. The aim of this study is the optimization of growth medium and physicochemical parameters effective on biosurfactant production in this isolate. Carbon and nitrogen sources, crude oil, NaCl concentration, temperature and time were investigated as the effective parameters on biosurfactant production by Bacillus licheniformis OT9 using experiment design (minitab.v16). The produced biosurfactant amount in each experiment was measured by the surface tension reduction (tensiometry). First, molasses and yeast extract were chosen qualitatively as the carbon and nitrogen sources. Then, using the Plackett–Burman design, 4 factors (nitrogen, temperature, time and crude oil) were determined as effective among the 6 factors mentioned above. After that, optimization of the effective parameters was carried out using Central Composite Design (CCD). The statistical method presented a quadratic equation as a model with the regression value of 94.13%. The optimum values were determined to be: yeast extract 1.12 gr⁄l , crude oil 1%, time 24 hours and temperature 55 c. These optimum values resulted in the maximum value of produced biosurfactant and surface tension reduction down to 39.5 mN⁄m .

نویسندگان

Mahsa Golghasemi Sorkhabi

Faculty of Petroleum and Chemical Engineering, University of Tabriz

Hassan Aghdasinia

Faculty of Petroleum and Chemical Engineering, University of Tabriz

Gholamreza Zarrini

Laboratory of Microbiology, Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz

Elnaz Ghasedi

Laboratory of Microbiology, Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz