TIM-3 expression was inhibited using mir-1285 in AML cell line

Introduction: Background: T-cell immunoglobulin mucin-3 (TIM-3) is expressed by monocytes,natural killer cells, and several cell subsets and leukemic myeloblasts. It can be one of thetherapeutic targets for malignant stem cells in AML and blockade of TIM-3 as regulatory receptormay be helpful for preventing CD8 cells exhaustion and for reducing leukemic stem cells (LSCs).MicroRNAs can affect AML progression through targeting different genes expressions.Materials Methods: Using bioinformatics tools, we predicted that mir-1285 could be able tosuppress TIM-3 expression. Human AML cell line HL-60 was cultured with RPMI 1640 suppliedwith 10% FBS. Phorbol myristate acetate (PMA) was used to induce the expression of TIM-3.These cells were transfected with mir-1285 mimic was used for transfection the cells and theexpression of TIM-3 was measured using both q-RT-PCR and flow-cytometry methods.Results: The results of our bioinformatics surveys were in accordance with our experiments whichconfirmed that miR-1285 is able to strongly silence TIM-3 expression (53.5% silencing) in HL-60cell line (p 0.003).Conclusion: According to our results, miR-1285 can suppress the expression of TIM-3 and can beconsidered for further studies about its potential effects on apoptosis, proliferation anddifferentiation of leukemic cells or for diagnosis of AML