Bone marrow on chip: Creation of 3D culture bioreactor over Stromal Cell to mimic HSCs niche

Introduction: Cord blood HSCs expansion in vitro is limited. 3D cell culture in bioreactor allowsmimicking the HSC niche. HSC expantion potency over stromal cells layer has been targeted indemineralized bone matrix as scaffold via 3D dynamic co-culture.Materials and methods: We have developed bioreactor made of PDMS.Cord blood CD34+HSCs cultured on the stromal layer under cytokine free condition for days. Flow cytometry,Colony assay and SEM morphlologically assessment on the result and initiative samples wereperformed.Results: At days co-culture, CD34+ cell number increment difference was statistically significantbetween dynamic and static groups. CD34+ HSCs expansion 4.7 folds increased in perfused cultureon day compared to statics culture at the same day.Conclusions: The ability of HSCs 3D culture expansion in bioreactor with stromal support overDBM scaffold could have emerged new approach for the current limitations of HSC transplantation