The Frequency of Reverse Transcriptase’s (RT) Genes in Escherichia coli Isolated of Urinary Tract Infections

سال انتشار: 1397
محل انتشار: اولین کنگره و نمایشگاه بین المللی علوم و تکنولوژی های نوین
کد COI اختصاصی: ICESIT01_204
زبان مقاله: انگلیسی
تعداد مشاهده: 300

نویسندگان

Department of genetic, Tonekabon branch, Islamic azad university, Tonekabon, Iran

چکیده

Background. The variable genome of Escherichia coli has caused the size of genome, MsDNAs, the Retrones, and the Reverse Transcriptase enzyme gene to vary in different strains of this bacterium. These genes called orphan genes that their function and origin is yet unknown. This study’s purpose is to determine the relative frequency of Reverse transcriptase in E. coli isolated from urinary tract infections. Methods. In this study 200 samples of urinary tract infections were collected from Tehran, Rasht, Tonekabon and Sari. DNA extraction from the specimens was performed using Boiling method and commercial kits (ROCHE). After designing and synthesizing specific primers, the gene was multiplied by PCR and its frequency was determined by SPSS software. Results. After performing statistical steps, out of 200 specimens, 27 samples had RT gene, and 31 were male (16%) and 169 were female (84%). Analysis by SPSS software showed that there is a significant difference between sexes about the presence or absence of the gene with 95% probability and 5% statistical level. Conclusion. The presence or absence of RT cannot be a reason for the pathogenicity of the E. coli. Analysis by SPSS software also showed that there is no significant difference between age groups regarding the presence or absence of the gene.

کلیدواژه ها

Escherichia coli, Orphan genes, RT (Reverse Transcriptase).

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