Use of Fat-Derived Stem Cells to Treat Emphysema

Background and Aim: We use this patient-naive pulmonary stem cell(NIPAM), an injectable hydrogel, in this project. Emphysema means thedestruction of the lung parenchyma, which results in the loss of lungelasticity and the loss of the lung s alveolar wall This reduces the amountof oxygen in the absorbing respiratory tract, all smokers develop tosome degrees of emphysema. In this condition, the wall of the alveoliis gradually eliminated and the individual suffering from shortness ofbreath, the progression of the disease depends on the amount of smoking.Methods: In this project, we introduce the cells from the culture mediuminto the colloid containing the nanogel precursor and additionallyadd some of the culture medium, which is called the encapsulationof pulmonary stem cells in the nanogel. Recent studies have beenconducted to treat this disease by lung stem cells for treating the diseaseon mice while using this nanogel as a scaffold to increase cellularconcentration and also prevent the development of an immune response.For treatment of cardiac MI, it has been used to encapsulate stem cells.A temperature-sensitive polymer was purified before being synthesizedby crystallization in a hexane and dried at room temperature. Then, thepolymer is synthesized continuously for 5 hours under the protection ofa nitrogen atmosphere at 70°C and used for the formulation of nanogel.Results: These nanogels have no adverse effect on the function and growth of stem cells, and the muscles are not repulsed after injection anddo not give rise to immune responses and if practiced, cause the lost lungparenchyma tissue to improve respiration.Conclusion: In this study, we have a new idea of temperature-sensitivepolymer to design an appropriate cell culture and cell-plate separationconstructed by tissue engineering without the need for a trypsin enzyme,and also the porosities of the polymeric structure can cause oxygen toenter and prevent the immune response.

کلیدواژه ها:

Emphysema ، Lung stem cells ، Encapsulation