The Effects of Mouse Embryonic Stem Cells Injection into the Morula on Pluripotency and Trophectoderm Genes Expression in MiceChimeric Blastocysts

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 331

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شناسه ملی سند علمی:

NSCMRMED03_336

تاریخ نمایه سازی: 30 دی 1397

چکیده مقاله:

Background and Aim: Chimera is an animal with two or more populationsof genetically distinct cells. Chimera has a significant impact onbiological and developmental researches. Nevertheless, chimeric animalexhibits less viability and more fetal and placental abnormalities thana normal animal. The aim of this study was to determine the effects ofmouse embryonic stem cells (mESCs) injection into the mouse embryoson some cell lineages genes expression in chimeric blastocystsMethods: In the first part of our experiment, the GFP-mESCs, 129/Sv, wereinjected into the in vivo-derived pre-compacted and compacted mouseembryos, C57BL/6, and the incorporation rate of the injected cells wasdetermined. In the second part, the expressions of inner cell mass (ICM)(Oct4 and Nanog) and trophectoderm (TE) (Tead4 andCdx2) lineagespecificgenes in the blastocysts derived from different approaches wereevaluated by real-time PCR and compared. The approaches were asfollows: i) in vivo-derived blastocysts (control); ii) injection of mESCs intothe subzonal space of in vivo-derived morula (chimeric blastocysts); iii)blastocysts derived from in vivo morula, and iv) blastocysts derived fromin vivo morula which received a sham injection.Results: The expressions of Oct4, Nanog and Tead4 in chimericblastocysts were lower than those in blastocysts of other groups (P<0.05). A significantly increased expression of Cdx2 was observed in the chimericblastocysts compared to sham (P> 0.05), while there was no significantdifference in Cdx2 expression of chimeric blastocysts with other groups.There was a significant difference in the production of chimeric blastocystusing two different stages of embryos so that subzonal injection of GFPmESCsat the pre-compacted embryos had greater advantages than acompacted stage in the production of chimeric blastocysts (P<0.05)Conclusion: Our data suggest that the formation of chemric blastocystsmay be negatively affected by alteration in the expression of genes inICM and TE. Furthermore, mESCs injection before embryo compactionincrease incorporation rate of the injected mESCs into the ICM andsubsequently the chance of mouse chimeric blastocyst formation.

کلیدواژه ها:

نویسندگان

Maryam Salimi

Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti. University of Medical Sciences,Tehran, Iran

Abolfazl Shirazi

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran- Research Institute of Animal Embryo Technology, ShahrekordUniversity, Shahrekord, Iran

Mohsen Norouzian

Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti. University of Medical Sciences,Tehran, Iran

Mohammad Mehdi Mehrazar

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran