Study of Expression of AKAP 4 and Catsper Genes as Motility Markers in Sperm Differentiation Pathway in Human Induced HairFollicle Stem Cells and Human Spermatogonial Stem Cells

Background and Aim: Nearly 72.4 million couples have infertilityproblems in the world. Men account for about 45-50% of infertility.Infertility in men has several causes that azoospermia is one of them.Nowadays, for the treatment of azoospermic patients, cell therapy is anew way. Infertile men can be used to induce follicular stem cells andspermatogonia to produce sperm. If the distinction is made into sperm,the genes of the mobility index should be expressed. The purpose of thisstudy is to evaluate the genes of Catsper, AKAP4, that contribute to spermmotility.Methods: In this study, follicular stem cells and spermatogonial wereused. Hair follicle cells were obtained by written consent from fertilizedmen who naturally had a child, and spermatogonial cells were obtainedby satisfying the testicles of biopsy patients. Samples were cultured in alaboratory environment. The expression of these genes was investigatedusing real-time PCR.Results: Stem cells grow in the culture medium. These cells weredifferentiated and altered by induction agents at specific time intervalsand ultimately resembled sperm cells, and these cells express the genes.These cells have tail and spindle shape head. Eventually, we prove theexpression of these genes by a real-time technique in different weeks.Conclusion: Stem cells exhibit the ability to convert into sperm-like cellsand can express some genes related to the mobility index. These genesexpressed in different Sperm production of stages and expression of themincreases gradually. Expression of these genes can be a reason for spermmaturation but it is better to study other sperm motility genes and haploiddependent genes.

کلیدواژه ها:

Azoospermia ، Infertility ، Real-time PCR ، Stem cell