Potential of Chrysin-Loaded Nanofibrous Mat for Wound Healing Applications Through its Antioxidant, Cytoprotective and Anti-Inflammatory Effects
- سال انتشار: 1397
- محل انتشار: سومین جشنواره ملی و کنگره بین المللی علوم و فناوری های سلول های بنیادی و پزشکی بازساختی
- کد COI اختصاصی: NSCMRMED03_307
- زبان مقاله: انگلیسی
- تعداد مشاهده: 391
نویسندگان
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
چکیده
Background and Aim: Chrysin (Chr), a naturally occurring flavone,with anti-inflammatory and anti-oxidant properties has been recentlyinvestigated for wound healing applications. However, some challengessuch as poor water solubility, low in vivo stability, rapid intestinal andhepatic metabolism, and low cellular uptake might limit its clinicalapplications. Considering to the high level of oxidative stress andpersistent inflammation related to delayed healing in chronic wounds,the present study was conducted to assess the bioactivity and potential ofencapsulated Chr into PCL/PEG nanofibrous mats as an antioxidant andanti-inflammatory agent.Methods: For this purpose, Chr-loaded PCL/PEG nanofibrous mats weresuccessfully fabricated by optimizing the electrospinning parameters andcharacterized using FE-SEM, FTIR, and UTM. MTT assay and FE-SEM wereused to evaluate the viability and attachment of Human foreskin fibroblastcells (HFF-1) cultured on the fibers for 1, 3, and 5 days, respectively.To measure the antioxidant activity of Chr-loaded nanofibrous mats, AnOxygen Radical Absorbance Capacity Assay (ORAC) was applied. Also,the cytoprotective potential of Chr-loaded PCL/PEG nanofibrous matscompared to PCL/PEG nanofibers in a situation of oxidative stress wasdetermined using MTT assay. The induction (relative mRNA levels) andrelease (protein expression levels) of three pro-inflammatory cytokines,IL-6 and IL-1β and TNF-α, were investigated in LPS-induced J774A1macrophages seeded on the nanofibers. Furthermore, the Griess reactionassay was used to measure nitrite production.Results: The randomly oriented structures of Chr-PCL/PEG nanofibersrevealed smooth surface and bead-free structures with a broad diameterdistribution (250-650 nm). Viability assay showed that HFF-1 havemore than 80% viability on Chr-loaded nanofibers. Also, the goodbiocompatibility of Chr-loaded PCL/PEG nanofibers was confirmedthrough cell adhesion assay. The capability of Chr-loaded nanofibersto maintain the viability of HFF-1 cells under an oxidative stresscondition demonstrated the antioxidant activities of the fibers. Also, Chrblendednanofibrous mats reduced overexpression of IL-6, IL-1β, TNF-αand excessive production of NO in J774A1 macrophages followingstimulation by LPS.کلیدواژه ها
Chrysin; Nanofiber; Wound healingمقالات مرتبط جدید
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