The Effect of Mesenchymal Stem Cell-Derived Microvesicles on Erythroid Differentiation of Umbilical Cord Blood-Derived CD34+ Cells

Background and Aim: Mesenchymal stem cells (MSCs) play an importantrole in the proliferation, apoptosis, and differentiation of hematopoieticstem cells (HSCs) in the bone marrow via cell-to-cell contact, as wellas secretion of cytokines and microvesicles (MVs). In this study, weinvestigated the effect of mesenchymal stem cell-derived microvesicles(MSC-MVs) on the erythroid differentiation of umbilical cord bloodderivedCD34+ cellsMethods: In this descriptive study, CD34+ cells were cultured with amixture of SCF (10 ng/mL) and rhEPO (5 U/mL) cytokines in the completeIMDM medium as a positive control group. Then, in MV1- and MV2-groups, MSC-MVs at 10 and 20 μg/mL concentrations were added. After72 hours, erythroid-specific markers (CD71 and CD235a) and genes(HBG1, GATA1, FOG1, and NFE2) were assessed by flow cytometry andqRT-PCR, respectivelyResults: The expression of specific markers of the erythroid lineages(CD71 and GPA) in the presence of different concentration of MSC-MVswere lower than that of the control group (P<0.001). Also, the expressionof specific genes of the erythroid lineages (NFE2, FOG1, GATA1, andHBG1) was investigated in comparison to the internal control (GAPDH).Among all of them, HBG1 and FOG1 genes were significantly decreasedto the control group (P<0.0001) but GATA1 and NFE2 gene expressionswere not significantConclusion: The results of this study showed that MSC-MVs decreasethe erythroid differentiation of umbilical cord blood-derived CD34+cells. Therefore, MSC-MVs play a key role in the regulation of normalerythropoiesis

کلیدواژه ها:

CD34+ cells ، Mesenchymal stem cells ، Extracellular vesicles ، Microvesicles ، Erythroid differentiation